Phospho-PDH E1 Alpha (Ser293) Recombinant antibody

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  WB analysis using 80688-2-RR

  Non-treated HeLa cells, phosphatase inhibitor and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 80688-2-RR (Phospho-PDH E1 Alpha (Ser293) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-PDH E1 Alpha (Ser293) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 80688-2-RR

Clone No.250640D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

P PDH E1 Alpha (Ser293), Phos PDH E1 Alpha (Ser293), Phos-PDH E1 Alpha (Ser293), Phospho-PDHA1 (Ser293), Phospho-Pyruvate Dehydrogenase α1 (Ser293)

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Tested Applications

• Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80688-2-RR targets Phospho-PDH E1 Alpha (Ser293) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08559
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)