Phospho-PDPK1 (Ser241) Polyclonal antibody

• 

  WB analysis using 29241-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

• 

  WB analysis using 29241-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-PDPK1 (Ser241) Polyclonal Antibody for WB, ELISA

Cat No. 29241-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

p PDPK1, p PDPK1 (Ser241), p PDPK1 Ser241, PDPK1 (Ser241), Phospho PDPK1

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Tested Applications

• Positive WB detected in: PDGF treated PC-3 cells, PDGF treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29241-1-AP targets Phospho-PDPK1 (Ser241) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: 3-phosphoinositide dependent protein kinase-1
• Calculated Molecular Weight: 556 aa, 63 kDa
• Observed Molecular Weight: 58-68 kDa
• GenBank Accession Number: BC012103
• Gene Symbol: PDPK1
• Gene ID (NCBI): 5170
• RRID: AB_2918256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15530
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Rep; A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism; Authors - Narendra Thapa