Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody

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• Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody (29546-1-AP)

Phospho-PERK/EIF2AK3 (Ser719) Polyclonal Antibody for WB, ELISA

Cat No. 29546-1-AP Copied!

Publications (66)

Reviews (1)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

p PERK/EIF2AK3, p PERK/EIF2AK3 (Ser719), p PERK/EIF2AK3 Ser719, PERK/EIF2AK3 (Ser719), Phospho PERK/EIF2AK3

• 

  WB analysis using 29546-1-AP

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 29546-1-AP

  Non-treated HeLa cells and Thapsigargin treated HeLa cells were subjected to SDS PAGE followed by western blot with 29546-1-AP (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Thapsigargin treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29546-1-AP targets Phospho-PERK/EIF2AK3 (Ser719) in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: eukaryotic translation initiation factor 2-alpha kinase 3
• Calculated Molecular Weight: 1116 aa, 125 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC126354
• Gene Symbol: PERK
• Gene ID (NCBI): 9451
• RRID: AB_2935416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NZJ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

Publications

What published studies show

Phospho-PERK/EIF2AK3 (Ser719) Polyclonal antibody (Cat# 29546-1-AP) has accumulated 66 citations across diverse research areas. Publications appear in high-impact journals including Nature, Science Advances, Cell Death & Disease, Cell Reports, Theranostics, Acta Pharmaceutica Sinica B, Free Radical Biology and Medicine, Journal of Biological Chemistry, Molecular Medicine, and International Journal of Oral Science. Research fields span endoplasmic reticulum stress, ferroptosis, apoptosis, cancer biology, neurodegeneration, inflammation, fibrosis, metabolic disorders, cardiovascular disease, renal injury, and environmental toxicology, with applications primarily in Western blot and immunohistochemistry.

Automatically generated from the 66 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nature; Ageing promotes metastasis via activation of the integrated stress response; Authors - Angana A H Patel
human	WB	Int J Oral Sci; Phenformin activates ER stress to promote autophagic cell death via NIBAN1 and DDIT4 in oral squamous cell carcinoma independent of AMPK; Authors - Dexuan Zhuang
		J Adv Res; Growth differentiation factor 15 impairs periodontal ligament stem cell proteostasis via integrated stress response to drive diabetic periodontitis.; Authors - Xiaoyu Yang
human	WB	J Adv Res; Impaired stemness in aging periodontal ligament stem cells is mediated by the progerin/endoplasmic reticulum stress/p53 axis; Authors - Xige Zhang
		Theranostics; Targeting the self-amplifying loop between ATF6 and SNAI1 to inhibit epithelial‒mesenchymal transition in fibrotic lesions; Authors - Tong Wu
human	WB	Acta Pharm Sin B; Phenylalanine deprivation inhibits multiple myeloma progression by perturbing endoplasmic reticulum homeostasis; Authors - Longhao Cheng

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product is a 5-star rating. The reviewer reported that the antibody performed well for Western Blot on HCT116 cells at a 1:1000 dilution. Overall, the feedback is positive, indicating reliable performance in the tested application.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Nathalie (Verified Customer) (09-26-2025)

Antibody worked well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT116 cells

Phospho-PERK/EIF2AK3 (Ser719) Antibody Western Blot validation (1:1000 dilution) in HCT116 cells (Cat no:29546-1-AP)