Phospho-POLR2A (Ser7) Polyclonal antibody

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• Phospho-POLR2A (Ser7) Polyclonal antibody (31139-1-AP)

Phospho-POLR2A (Ser7) Polyclonal Antibody for WB, ELISA

Cat No. 31139-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

p POLR2A, p POLR2A (Ser7), p POLR2A Ser7, Phospho POLR2A, Phospho POLR2A (Ser7)

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  WB analysis using 31139-1-AP

  Phosphatase inhibitor treated HeLa cells, λ phosphatase treated HeLa cells, and non-treated HeLa cells, were subjected to SDS PAGE followed by western blot with 31139-1-AP (Phospho-POLR2A (Ser7) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31139-1-AP targets Phospho-POLR2A (Ser7) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Calculated Molecular Weight: 217 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_000937
• Gene Symbol: POLR2A
• Gene ID (NCBI): 5430
• RRID: AB_3669869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24928
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.

Publications

What published studies show

The Phospho-POLR2A (Ser7) Polyclonal antibody (Cat# 31139-1-AP) has 1 total citation. It appears in Biochimica et Biophysica Acta (BBA) – Gene Regulatory Mechanisms, a journal focused on gene regulation. The cited study investigates RNA polymerase II transcription elongation in Saccharomyces cerevisiae, placing the research field in molecular biology and transcription regulation. The antibody was used in Western blot (WB) for that application.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Biochim Biophys Acta Gene Regul Mech; Growth rate is related to elongation of RNA polymerase II transcription in Saccharomyces cerevisiae; Authors - A I Garrido-Godino