Tested Applications
| Positive WB detected in | pervanadate treated HeLa cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81180-2-RR targets Phospho-Paxillin (Tyr118) in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | paxillin |
| Calculated Molecular Weight | 591 aa, 65 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC136787 |
| Gene Symbol | Paxillin |
| Gene ID (NCBI) | 5829 |
| RRID | AB_3670493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49023 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Paxillin (p68-kD) is one of the integrin assembly proteins and can interact directly with several integrin assembly proteins, including vinculin, talin, β1 integrin, focal adhesion kinase (FAK), c-Src and Csk. Integrin-mediated tyrosine phosphorylation also enables paxillin to interact with various signaling molecules: tyrosine residues (Y) 31 and 118 being especially predominant targets of phosphorylation by kinases and creating binding sites for the SH2 domain of adaptor protein Crk. Thus, paxillin plays apivotal role in cell adhesion, migration and further oncogenic transformation. (PMID: 11774284)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-Paxillin (Tyr118) antibody 81180-2-RR | Download protocol |
| WB protocol for Phospho-Paxillin (Tyr118) antibody 81180-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-Paxillin (Tyr118) Recombinant monoclonal antibody (Cat# 81180-2-RR) has 2 citations. One appeared in Cell Death Discovery, investigating the STAT3-PXN positive feedback loop in glioblastoma and YB-1 ubiquitination regulation. The other was published in Biochemical and Biophysical Research Communications, examining ITGA5-driven epithelial-mesenchymal transition and metabolic adaptation in thyroid cancer. The research fields span neuro-oncology (glioblastoma) and thyroid cancer biology, with applications in IF and WB.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Deciphering the STAT3-PXN positive feedback loop in GBM, IDH-wildtype: transcriptional regulation and inhibition of YB-1 ubiquitination. | ||
Biochem Biophys Res Commun Single-cell and bulk transcriptomic analyses reveal ITGA5-driven epithelial-mesenchymal transition and metabolic adaptation in thyroid cancer |
Reviews
What customers say
One reviewer used this antibody for Western Blot on A549 cells at a 1:5000 dilution, incubating overnight at 4 °C, followed by a donkey anti-rabbit secondary antibody (Alexa Fluor 800) at 1:20000 for 2 hours at room temperature. The antibody detected two blurry bands between 50–75 kDa. The reviewer rated the product 3 out of 5, suggesting the signal specificity and band sharpness could be improved. Overall, the antibody functioned as expected for WB but produced less crisp results than ideal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Angie (Verified Customer) (04-14-2026) | The antibody detected two blurry bands between 50-75 kDa, used at dilution of 1:5000 incubated overnight at 4 °C followed by secondary antibody donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution, incubated 2 hour at room temperature.
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