Phospho-RIPK1 (Ser166) Recombinant monoclonal antibody

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• Phospho-RIPK1 (Ser166) Recombinant monoclonal antibody (84036-4-RR)

Phospho-RIPK1 (Ser166) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84036-4-RR

Clone No.243009B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RIPK1, EC:2.7.11.1, Receptor interacting serine/threonine protein kinase 1, Receptor-interacting protein 1, Receptor-interacting serine/threonine-protein kinase 1

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  WB analysis of HT-29 using 84036-4-RR

  Non-treated HT-29 cells and TNF alpha treated HT-29 cells were subjected to SDS PAGE followed by western blot with 84036-4-RR (Phospho-RIPK1 (Ser166) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: TNF alpha treated HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84036-4-RR targets Phospho-RIPK1 (Ser166) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_003804
• Gene Symbol: RIPK1
• Gene ID (NCBI): 8737
• RRID: AB_3743515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13546
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIPK1, a 74 kDa protein, is composed of a N-terminal kinase domain, an intermediate domain (containing the RIP homotypic interaction motif, RHIM) and a C-terminal death domain. Stimulation of cells with TNFα can promote distinct cell death pathways, including RIPK1-independent apoptosis, necroptosis, and RIPK1-dependent apoptosis (RDA). TNFα induces cell necroptosis and the phosphorylation of RIPK1 at the Ser166 residue i.e. p-RIPK1 (Ser166), both of which can be effectively inhibited by Nec-1. Therefore, p-RIPK1 (Ser166) is considered a biomarker for the activation of RIPK1 kinase and necroptosis (PMID: 31440386, PMID: 29891719).