Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal antibody

• 

  WB analysis using 29223-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

• 

  WB analysis using 29223-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal Antibody for WB, ELISA

Cat No. 29223-1-AP

Publications(13)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Synonyms

p S6Ribosomal protein, p S6Ribosomal protein (Ser235/236), p S6Ribosomal protein Ser235/236, Phospho RPS6, Phospho RPS6 (Ser235/236)

---

Tested Applications

• Positive WB detected in: PDGF treated NIH/3T3 cells, λ phosphatase treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 12 publications below
• IF: See 1 publications below

Product Information

29223-1-AP targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: ribosomal protein S6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC000524
• Gene Symbol: RPS6
• Gene ID (NCBI): 6194
• RRID: AB_2918253
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62753
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Human	WB	Sci Rep; STK11 genetic alterations in metastatic EGFR mutant lung cancer; Authors - Dandan Yin
mouse	WB	ACS Omega; ROS-responsive MSC-derived Exosome Mimetics Carrying MHY1485 Alleviate Renal Ischemia Reperfusion Injury through Multiple Mechanisms; Authors - Zhiyu Qian
human	WB	Int J Med Sci; PRMT4 promotes hepatocellular carcinoma progression by activating AKT/mTOR signaling and indicates poor prognosis.; Authors - Peng Du
human	WB	Metabolism; Anti-sarcopenic effects of active vitamin D through modulation of anabolic and catabolic signaling pathways in human skeletal muscle: A randomized controlled trial; Authors - Tetsuya Kawahara
human	WB	Transl Oncol; Next-generation sequencing in early-stage multiple primary lung cancer: The prognostic significance of genomic accumulation status and BCL2L11del; Authors - Mu-Ting Wang
human	WB	Mol Carcinog; LINC02154 Promotes Esophageal Squamous Cell Carcinoma Progression via the PI3K-AKT-mTOR Signaling Pathway by Interacting With IGF2BP2; Authors - Mingyuan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Morgane (Verified Customer) (09-15-2025)

Very good anti-phospho-antibody, very specific to RPS6

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

Phospho-S6 Ribosomal protein (Ser235/236) Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:29223-1-AP)

FH

Reyes (Verified Customer) (02-14-2025)

pS6 worked perfectly (in green) showing the mTOR activation in my neurons (in red NeuN) and Balloon Cells on human brain FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Phospho-S6 Ribosomal protein (Ser235/236) Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:29223-1-AP)