Tested Applications
| Positive WB detected in | 10 % fetal bovine serum treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29220-1-AP targets Phospho-S6 Ribosomal protein (Ser240/244) in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ribosomal protein S6 |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | NM_001010 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_2918252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomal protein S6 (RPS6), also named as Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-S6 Ribosomal protein (Ser240/244) antibody 29220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-S6 Ribosomal protein (Ser240/244) Polyclonal antibody (Cat# 29220-1-AP) has 4 total citations. These appear in Biochimica et Biophysica Acta (Molecular Cell Research), Biochimie, Veterinary Microbiology, and Frontiers in Neuroscience. The associated research fields include radiation-induced lung injury and autophagy/mTOR signaling, doxorubicin-induced cellular senescence, viral mTOR signaling disruption, and epilepsy-associated cortical malformations.
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Cell Res IL-17A's role in exacerbating radiation-induced lung injury: Autophagy impairment via the PP2A-mTOR pathway | ||
Biochimie Doxorubicin-induced senescence is modulated by the eukaryotic release factor 3a and its polyglycine expansion in HCT116 cells | ||
Vet Microbiol Porcine reproductive and respiratory syndrome virus nsp2-related proteins induce host translational arrest by specifically impairing the mTOR signaling cascade | ||
Front Neurosci Identification of microglia-associated hub genes and a CX3CR1/P2RY12 two-gene diagnostic model for cross-subtype epilepsy-associated cortical malformations: integrated transcriptomic analysis with external validation. |
