Phospho-SHP2 (Tyr580) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81219-2-RR (same clone as 81219-2-PBS)

  Non-treated C6 cells and PDGF (HZ-1215) treated C6 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81219-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81219-2-RR (same clone as 81219-2-PBS)

  Non-treated NIH/3T3 cells and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81219-2-PBS in a different storage buffer formulation.

Phospho-SHP2 (Tyr580) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 81219-2-PBS

Clone No.242434E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

P SHP2 (Tyr580), Phospho SHP-2 (Tyr580), Phospho-SHP2, Phospho-SHP-2, Phospho-SHP-2 (Tyr580)

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Product Information

81219-2-PBS targets Phospho-SHP2 (Tyr580) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein tyrosine phosphatase, non-receptor type 11
• Calculated Molecular Weight: 597 aa, 68 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC008692
• Gene Symbol: PTPN11
• Gene ID (NCBI): 5781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q06124
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)