Tested Applications
| Positive WB detected in | IFN gamma and LPS treated THP-1 cells |
| Positive IF/ICC detected in | IFN alpha treated HeLa cells |
| Positive FC (Intra) detected in | IFN gamma treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28979-1-AP targets Phospho-STAT1 (Tyr701) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, rabbit, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | signal transducer and activator of transcription 1, 91kDa |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 84-91 kDa |
| GenBank Accession Number | BC002704 |
| Gene Symbol | STAT1 |
| Gene ID (NCBI) | 6772 |
| RRID | AB_2935412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42224 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-AP | Download protocol |
| WB protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
28979-1-AP is a STAT1 antibody with 25 total citations. Journals include Nature Communications, Molecular Neurodegeneration, Cell Death Discovery, mBio, Journal of Virology, Phytotherapy Research, Life Sciences, Scientific Reports, Veterinary Research, Molecular Immunology, Journal of Clinical Medicine, and others. Research fields span JAK-STAT signaling, type I interferon responses, ferroptosis, viral infections (PRRSV, TGEV, FIPV, ASfV, HBV), liver disease, neurodegeneration, cancer immunology, rheumatoid arthritis, and dry eye pathogenesis.
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice. | ||
Nat Commun Proteome-wide ligandability maps of drugs with diverse cysteine-reactive chemotypes | ||
Cell Death Discov RUNX1 restrains STAT1-GITRL signaling to shape an immunosuppressive CRC microenvironment.
| ||
J Transl Med Pretreatment of UC-MSCs with IFN-α2 improves treatment of liver fibrosis by recruiting neutrophils | ||
Phytother Res Coptisine Improves Liver Inflammation in Sepsis by Regulating STAT1/IRF1/GPX4 Signaling-Mediated Kupffer Cells Ferroptosis | ||
Life Sci Cadmium reprograms type-I interferon signaling to promote Japanese encephalitis virus infection in neuronal cells. |
Reviews
What customers say
One verified user rated this antibody 5/5 stars for Western Blot (lot 00110127, dilution 1:3000). The reviewer described it as a "great working antibody" with clean background, confirming reliable performance in their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Agnieszka (Verified Customer) (02-28-2023) | great working antibody, gives the background
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