Phospho-STAT3 (Ser727) Recombinant monoclonal antibody

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• Phospho-STAT3 (Ser727) Recombinant monoclonal antibody (80199-2-RR)

Phospho-STAT3 (Ser727) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA, ChIP-qPCR

Cat No. 80199-2-RR

Clone No.240778A12

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC (Intra), ELISA, ChIP-qPCR

Synonyms

p STAT3, p STAT3 (Ser727), p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)

• 

  WB analysis of HeLa using 80199-2-RR

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 80199-2-RR (Phospho-STAT3 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

• 

  FC experiment of HEK-293T using 80199-2-RR

  1x10^6 Calyculin A treated HEK-293T cells were intracellularly stained with 0.25 ug Stat3 Recombinant Antibody (80199-2-RR, Clone:240778A12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

• 

  ChIP experiment of HepG2 using 80199-2-RR

  Chromatin was prepared from HepG2 cells (starved overnight) either non-treated or treated with IL-6 (100ng/mL, 30min). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Phospho-STAT3 (Ser727) (80199-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive FC (Intra) detected in: Calyculin A treated HEK-293T cells
• Positive ChIP-qPCR detected in: IL-6 (100ng/mL,30min) HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHIP-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80199-2-RR targets Phospho-STAT3 (Ser727) in WB, IHC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Calculated Molecular Weight: 770 aa, 88 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC000627
• Gene Symbol: STAT3
• Gene ID (NCBI): 6774
• RRID: AB_3670470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P40763
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Neuropharmacology; Pantothenic acid-mediated inhibition of microglial inflammation via the JAK2/STAT3 pathway enhances motor function recovery after Spinal cord injury; Authors - Yuepeng Fang
	WB,IHC	J Mol Histol; CSF2RA promotes gastric cancer progression through activation of the JAK2/STAT3 signaling pathway; Authors - Mengjie Li
human	WB	Indian J Hematol Blood Transfus; SAR317461 Inhibit JAK2/STAT3 Phosphorylation for Enhanced Anti-leukemia Activity.; Authors - Xiao-Yu Yang
mouse	WB	Front Endocrinol (Lausanne); Cangfu Daotan decoction treats PCOS-IR through the IL6/JAK2/STAT3/FOXO4 signaling pathway.; Authors - Wenhan Ju
		Cell Death Discov; MFAP2 secreted by TGF-β1-induced cancer-associated fibroblast cells promotes gastric cancer peritoneal metastasis through Src-STAT3-PTK7 axis.; Authors - Yuen Tan
mouse	WB	Genes Immun; Secreted SULF1 protein modulates CD8 + T cell exhaustion by promoting TAM polarization in gastric cancer.; Authors - Xiaodan Lu