Phospho-STAT5A (Ser726)/STAT5B (Ser731) Polyclonal antibody

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  WB analysis using 28951-1-AP

  Non-treated TF-1 and GM-SCF treated TF-1 cells were subjected to SDS PAGE followed by western blot with 28951-1-AP (Phospho-STAT5A(Ser726)/STAT5B (Ser731) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-STAT5A (Ser726)/STAT5B (Ser731) Polyclonal Antibody for WB, ELISA

Cat No. 28951-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho-STAT5A(Ser726)/STAT5B (Ser731), STAT5A, STAT5, MGF

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Tested Applications

• Positive WB detected in: GM-CSF treated TF-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28951-1-AP targets Phospho-STAT5A (Ser726)/STAT5B (Ser731) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: signal transducer and activator of transcription 5A
• Calculated Molecular Weight: 794 aa, 92 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC027036
• Gene Symbol: STAT5A
• Gene ID (NCBI): 6776
• RRID: AB_2918220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42229
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. This antibody can recognize the phosphorylation of STAT5A at Ser726 and the phosphorylation of STAT5B at Ser731.

Publications

Species	Application	Title
human	WB	Endocrinology; A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration; Authors - Ruonan Li
human	WB	PeerJ; RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA; Authors - Jin Fan