Phospho-STK11/LKB1 (Thr189) Polyclonal antibody

Phospho-STK11/LKB1 (Thr189) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28965-1-AP

Synonyms

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11



Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells, UV treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

28965-1-AP targets Phospho-STK11/LKB1 (Thr189) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name serine/threonine kinase 11
Calculated molecular weight 49 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC007981
Gene symbol STK11
Gene ID (NCBI) 6794
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Liver kinase 1 (LKB1 also known as STK11) is a tumor suppressor gene that is inactivated by bi-allelic mutation in non-small cell lung cancer (NSCLC), malignant melanoma and cervical cancer. LKB1 protein has a central kinase domain, two N-terminal nuclear leading sequences, and a C-terminal regulatory motif. The protein also contains a CAAX motif at C-terminal end and can be farnesylated. The formation of an LKB1/Strad/MO25 complex is essential for LKB1's kinase activity, and there are at least 13 known substrates of LKB1, which includes AMPKs, BRSKs, and MARKs. LKB1 is the major kinase that phosphorylates the AMPK activation loop in conditions of energy stress. (PMID: 25679014, PMID: 19629071)

Protocols

Product Specific Protocols
WB protocol for Phospho-STK11/LKB1 (Thr189) antibody 28965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols