Tested Applications
| Positive WB detected in | HEK-293T cells, λ phosphatase treated HEK-293T cells |
This antibody is equivalent to Ser412 in mice.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30895-1-AP targets Phospho-TAK1 (Ser439) in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 7 |
| Calculated Molecular Weight | 579 aa, 64 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC017715 |
| Gene Symbol | TAK1 |
| Gene ID (NCBI) | 6885 |
| RRID | AB_3669777 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43318 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. TAK1 has been identified as a crucial regulatory component of MAPK and nuclear factor-κB (NF-κB) signaling pathways, which play a key role in lipid metabolism and inflammation. Activated TAK1 then phosphorylates downstream substrates to spark the NF-κB and MAPK signaling pathways. Hence, phospho-TAK1 confers most of its regulatory functions, and inhibiting the hyperactivation of TAK1 should be a promising NASH(Nonalcoholic steatohepatitis ) therapy. (PMID: 34146477) This antibody is equivalent to Ser412 in mice.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TAK1 (Ser439) antibody 30895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
