Phospho-TAU (Ser199) Recombinant monoclonal antibody, PBS Only

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• Phospho-TAU (Ser199) Recombinant monoclonal antibody, PBS Only (86995-1-PBS)

Phospho-TAU (Ser199) Uni-rAb^® Recombinant Antibody for WB, IF-P, Indirect ELISA

Cat No. 86995-1-PBS

Clone No.252075E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, Indirect ELISA

Synonyms

p TAU, p TAU (Ser199), p TAU Ser199, phos TAU, Phospho TAU

• 

  WB analysis using 86995-1-RR (same clone as 86995-1-PBS)

  Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 86995-1-RR (Phospho-TAU (Ser199) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 86995-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 86995-1-RR (same clone as 86995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-TAU (Ser199) antibody (86995-1-RR, Clone: 252075E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86995-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

86995-1-PBS targets Phospho-TAU (Ser199) in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 37-46, 79-81 kDa
• Observed Molecular Weight: 35-60 kDa
• GenBank Accession Number: BC000558
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• RRID: AB_3745263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.