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Phospho-TAU (Ser214) Recombinant monoclonal antibody, PBS Only (87043-3-PBS)

Phospho-TAU (Ser214) Recombinant monoclonal antibody, PBS Only

Name: Phospho-TAU (Ser214) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87043-3-PBS-100UG

Phospho-TAU (Ser214) Uni-rAb^® Recombinant Antibody for WB, IF-P, Indirect ELISA

Cat No. 87043-3-PBS

Clone No. 252082D9

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

P TAU (Ser214), phos TAU, Phospho TAU (Ser214), Phospho-TAU Ser214, phos-TAU

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

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Filter: AllWBIF-P

WB analysis of mouse brain using 87043-3-RR (same clone as 87043-3-PBS)
Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 87043-3-RR (Phospho-TAU (Ser214) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87043-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 87043-3-RR (Phospho-TAU (Ser214) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87043-3-PBS in a different storage buffer formulation.
WB analysis of rat brain using 87043-3-RR (same clone as 87043-3-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 87043-3-RR (TAU-Ser214 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87043-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 87043-3-RR (same clone as 87043-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-TAU (Ser214) antibody (87043-3-RR, Clone: 252082D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87043-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Phospho-TAU (Ser214) antibody (87043-3-RR, Clone: 252082D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87043-3-PBS in a different storage buffer formulation.

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Overview

Tested Applications
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Product Information

87043-3-PBS targets Phospho-TAU (Ser214) in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	microtubule-associated protein tau
Calculated Molecular Weight	37-46, 79-81 kDa
Observed Molecular Weight	45-60 kDa
GenBank Accession Number	BC000558
Gene Symbol	TAU
Gene ID (NCBI)	4137
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P10636
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Phospho-Tau (Ser214) is a PKA-dependent phosphorylation site critical for Alzheimer's disease (AD) pathology. Ser214 hyperphosphorylation disrupts microtubule binding, promotes neurofibrillary tangle formation, and serves as a highly specific biomarker for AD and related tauopathies.