Phospho-TAU (Ser396) Recombinant monoclonal antibody

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  WB analysis using 87029-11-RR

  Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 87029-11-RR (Phospho-TAU (Ser396) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  IF Staining of mouse brain using 87029-11-RR

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated mouse brain tissue using Phospho-TAU (Ser396) antibody (87029-11-RR, Clone: 252170E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Phospho-TAU (Ser396) Uni-rAb^® Recombinant Antibody for WB, IF-P, ELISA

Cat No. 87029-11-RR

Clone No.252170E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

p TAU, p TAU (Ser396), p TAU Ser396, phos TAU, Phospho TAU

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF-P detected in: λ phosphatase treated mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87029-11-RR targets Phospho-TAU (Ser396) in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: microtubule-associated protein tau
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 50-80 kDa
• GenBank Accession Number: NM_016835
• Gene Symbol: TAU
• Gene ID (NCBI): 4137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10636
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tau is a 441-amino acid hydrophilic protein sub-classified into 4 domains. The amino acids Thr181, Thr205 and Thr217 are associated with the early stages in the development of AD, while amino acid residues Ser262, Ser396 and Ser404 are associated with formation of aggregates.