Phospho-TBK1 (Ser172) Recombinant antibody

Phospho-TBK1 (Ser172) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2K2

Reviews (1)

Cat no : 82383-1-RR

Synonyms

FLJ11330, NAK, NF kappa B activating kinase, Phospho-TBK1 (Ser172), T2K, TANK binding kinase 1, TBK1

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Validation Data Gallery

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  WB analysis of HeLa using 82383-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82383-1-RR (Phospho-TBK1 (Ser172) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control.

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Tested Applications

• Positive WB detected in: Calyculin A treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

82383-1-RR targets Phospho-TBK1 (Ser172) in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: TANK-binding kinase 1
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC034950
• Gene Symbol: TBK1
• Gene ID (NCBI): 29110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy.

Publications

Species	Application	Title
human,mouse	WB	Signal Transduct Target Ther; The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation; Authors - Weijie Ouyang
mouse	WB	Eur J Pharmacol; Nitisinone attenuates cartilage degeneration and subchondral osteoclastogenesis in osteoarthritis and concomitantly inhibits the cGAS/STING/NF-κB pathway; Authors - Tao Yang
		Cell Rep; Non-canonical isoforms of the mRNA polyadenylation factor WDR33 regulate STING-mediated immune responses; Authors - Lizhi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (09-27-2023)

Western blot using p-TBK1 antibody at 1:5000 dilution overnight at 4ºC. Samples are two breast cancer (BC) cell lines, ran in duplicate. Ponceau staining is shown as total protein loading control. A more prominent band can be seen below 100 kDa, and after more exposure, another band at around 80 kDa can be observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Triple negative breast cancer cell lines

Phospho-TBK1 (Ser172) Antibody Western Blot validation (1:5000 dilution) in Triple negative breast cancer cell lines (Cat no:82383-1-RR)