Phospho-TSC2 (Ser1387) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80553-2-RR (same clone as 80553-2-PBS)

  Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80553-2-RR (Phospho-TSC2 (Ser1387) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody (66305-1-Ig) as the loading control. This data was developed using the same antibody clone with 80553-2-PBS in a different storage buffer formulation.

Phospho-TSC2 (Ser1387) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 80553-2-PBS

Clone No.242665D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

TSC2, Tuberin, TSC4, Tuberin/TSC2, tuberous sclerosis 2

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Product Information

80553-2-PBS targets Phospho-TSC2 (Ser1387) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tuberous sclerosis 2
• Calculated Molecular Weight: 1807 aa, 201 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC150300
• Gene Symbol: TSC2
• Gene ID (NCBI): 7249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49815
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. AMPK can phosphorylate and activate the Ser1387 site of TSC2.(PMID: 14651849, 12172553, PMID: 23244622)