Phospho-TSC2 (Ser939) Recombinant monoclonal antibody

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  WB analysis using 81654-1-RR

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81654-1-RR (Phospho-TSC2 (Ser939) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control.

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  FC experiment of HEK-293 using 81654-1-RR

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.06 ug Phospho-TSC2 (Ser939) Recombinant antibody (81654-1-RR, Clone:2B18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-TSC2 (Ser939) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 81654-1-RR

Clone No.2B18

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Phospho TSC2 (Ser939), Phospho TSC2, p TSC2 Ser939, p TSC2 (Ser939), p TSC2

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Tested Applications

• Positive WB detected in: Calyculin A treated HEK-293 cells
• Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

81654-1-RR targets Phospho-TSC2 (Ser939) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tuberous sclerosis 2
• Calculated Molecular Weight: 1807 aa, 201 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC150300
• Gene Symbol: TSC2
• Gene ID (NCBI): 7249
• RRID: AB_3085356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49815
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (PMID: 12150915).