Tested Applications
| Positive WB detected in | PDGF treated PC-3 cells, PDGF treated NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29000-1-AP targets Phospho-TSC2 (Thr1462) in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tuberous sclerosis 2 |
| Calculated Molecular Weight | 1807 aa, 201 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC150300 |
| Gene Symbol | TSC2 |
| Gene ID (NCBI) | 7249 |
| RRID | AB_2918227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49815 |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TSC2 (Thr1462) antibody 29000-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-TSC2 (Thr1462) Polyclonal antibody (Cat# 29000-1-AP) has 3 total citations. The cited journals are Research, Frontiers in Cell and Developmental Biology, and Virology Journal. Research fields span prostate cancer tumorigenesis and metastasis, high-power microwave effects on corneal epithelial cells, and apoptosis/autophagy in herpes simplex keratitis. All citations used the antibody for Western blot in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Research (Wash D C) The FTO Mediated N6-Methyladenosine Modification of DDIT4 Regulation with Tumorigenesis and Metastasis in Prostate Cancer | ||
Front Cell Dev Biol The effect of 4.3 GHz high-power microwave exposure on human corneal epithelial cells. | ||
Virol J SGK2 mediates apoptosis in herpes simplex keratitis by suppressing protective autophagy via the mTOR pathway. |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The reviewer used it for Western Blot at a 1:1000 dilution on mouse brain lysate and reported successful detection of endogenous pT1462 TSC2 at the expected molecular weight of approximately 200 kDa. However, they noted the presence of some possible non-specific bands, suggesting the antibody performs adequately but may benefit from optimization to reduce background.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cali (Verified Customer) (01-21-2025) | It is okay to detect endogenous pT1462 TSC2 with the right molecular weight (about 200 kd), although some possible non-specific bands exist.
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