Phospho-ULK1 (Ser556) Recombinant antibody, PBS Only

Phospho-ULK1 (Ser556) Recombinant Antibody for WB, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F5

Cat no : 80218-1-PBS

Synonyms

ATG1, FLJ38455, FLJ46475, KIAA0722, Phospho-ULK1 (Ser556), ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

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Validation Data Gallery

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  WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

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  WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 80218-1-RR (same clone as 80218-1-PBS)

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-ULK1 (Ser556) (80218-1-RR, Clone:2F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80218-1-PBS targets Phospho-ULK1 (Ser556) in WB, FC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-51-like kinase 1 (C. elegans)
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)