Phospho-VPS34 (Thr159) Polyclonal antibody

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• Phospho-VPS34 (Thr159) Polyclonal antibody (29675-1-AP)

Phospho-VPS34 (Thr159) Polyclonal Antibody for WB, ELISA

Cat No. 29675-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

Phospho VPS34 (Thr159), Phospho-VPS34, Phospho-VPS34 Thr159, p-VPS34, p-VPS34 (Thr159)

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  WB analysis using 29675-1-AP

  Non-treated SH-SY5Y cells, phosphatase inhibitor treated SH-SY5Y cells cells and λ phosphatase treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29675-1-AP (Phospho-VPS34 (Thr159) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, λ phosphatase treated SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29675-1-AP targets Phospho-VPS34 (Thr159) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phosphoinositide-3-kinase, class 3
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_002647
• Gene Symbol: VPS34
• Gene ID (NCBI): 5289
• RRID: AB_3669693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NEB9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS34 phosphorylates phosphatidylinositol to produce PtdIns3P and is the progenitor of the phosphoinositide 3-kinase (PI3K) family. VPS34 has a simpler domain organization than class I PI3Ks, which belies the complexity of its quaternary organization, with the enzyme always functioning within larger assemblies. (PMID: 30397185)

Publications

What published studies show

The Phospho-VPS34 (Thr159) polyclonal antibody (Cat# 29675-1-AP) has 3 citations. These appear in Phytomedicine (cerebral ischemia-reperfusion injury and PI3K/Akt/GSK3β signaling), European Journal of Medicinal Chemistry (pancreatic cancer and FAK-targeted drug discovery), and Neurochemical Research (neonatal hippocampal neurotoxicity). All citations used WB in human or mouse samples, spanning fields of neuroprotection, oncology, and developmental neuroscience.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Phytomedicine; Nuciferine alleviates cerebral ischemia-reperfusion injury by inhibiting mPTP opening via activating the phosphatidylinositol 3-kinase/protein kinase B/glycogen synthase kinase 3 beta pathway.; Authors - Hehe Wang
human	WB	Eur J Med Chem; Discovery of a potent anticancer agent against pancreatic ductal adenocarcinoma targeting FAK with DFG-out state and JAK/Aurora kinases; Authors - Rong-Hong Zhang
mouse	WB	Neurochem Res; Single-Nucleus Transcriptomic Landscape of Neonatal Mice Hippocampus During Sevoflurane-Induced Neurotoxicity.; Authors - Yanan Li