Phospho-Vimentin (Ser56) Recombinant monoclonal antibody

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  WB analysis using 87149-1-RR

  Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87149-1-RR (Phospho-Vimentin (Ser56) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

• 

  WB analysis using 87149-1-RR

  Non-treated Jurkat cells and paclitaxel treated Jurkat cells were subjected to SDS PAGE followed by western blot with 87149-1-RR (Phospho-Vimentin (Ser56) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-Vimentin (Ser56) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87149-1-RR

Clone No.252334B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho Vimentin, Phospho Vimentin (Ser56), Phospho-VIM (Ser56), Phospho-Vimentin, Phospho-Vimentin(Ser56)

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Tested Applications

• Positive WB detected in: paclitaxel treated Jurkat cells, paclitaxel treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87149-1-RR targets Phospho-Vimentin (Ser56) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: vimentin
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: NM_003380
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• ENSEMBL Gene ID: ENSG00000026025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08670
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Phospho-Vimentin (Ser56) is a phosphorylated form of the intermediate filament protein Vimentin that plays a significant role in regulating cellular functions such as smooth muscle contraction, cell migration, and mitosis. Phosphorylation of Vimentin at Ser-56 has been found to regulate vimentin depolymerization and the spatial reorientation of vimentin filaments, which modulates intercellular force transmission and contraction in smooth muscle. (PMID: 27457922)