Phospho-XIAP (Ser87) Polyclonal antibody

Phospho-XIAP (Ser87) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28791-1-AP

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, Phospho-XIAP (Ser87), S87, Ser87, X linked IAP, XIAP, XIAP (Ser87), XIAP S87, XIAP Ser87, XLP2

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Validation Data Gallery

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  WB analysis using 28791-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28791-1-AP (Phospho-XIAP (Ser87) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PDGF treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28791-1-AP targets Phospho-XIAP (Ser87) in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: X-linked inhibitor of apoptosis
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC032729
• Gene Symbol: XIAP
• Gene ID (NCBI): 331
• RRID: AB_2881213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. XIAP is directly phosphorylated by PKC and that XIAP phosphorylated at Ser87 by PKC is resistant to degradation, protecting cells from apoptosis-inducing stress.