Phospho-XIAP (Ser87) Polyclonal antibody

Phospho-XIAP (Ser87) Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 28791-1-AP

Synonyms

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, Phospho-XIAP (Ser87), S87, Ser87, X linked IAP, XIAP, XIAP (Ser87), XIAP S87, XIAP Ser87, XLP2



Tested Applications

Positive WB detected inPDGF treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

28791-1-AP targets Phospho-XIAP (Ser87) in WB,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name X-linked inhibitor of apoptosis
Calculated molecular weight 60 kDa
Observed molecular weight57 kDa
GenBank accession numberBC032729
Gene symbol XIAP
Gene ID (NCBI) 331
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. XIAP is directly phosphorylated by PKC and that XIAP phosphorylated at Ser87 by PKC is resistant to degradation, protecting cells from apoptosis-inducing stress.

Protocols

Product Specific Protocols
WB protocol for Phospho-XIAP (Ser87) antibody 28791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols