Phospho-c-MYC (Thr58) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 87268-1-RR (same clone as 87268-1-PBS)

  Non-treated and MG132 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 87268-1-RR (Phospho-c-MYC (Thr58) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 87268-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87268-1-RR (same clone as 87268-1-PBS)

  Non-treated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 87268-1-RR (Phospho-c-MYC (Thr58) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 87268-1-PBS in a different storage buffer formulation.

Phospho-c-MYC (Thr58) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87268-1-PBS

Clone No.252290C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

MYC, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, c-Myc

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Product Information

87268-1-PBS targets Phospho-c-MYC (Thr58) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-myc myelocytomatosis viral oncogene homolog (avian)
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC000141
• Gene Symbol: MYC
• Gene ID (NCBI): 4609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01106
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)