Phospho-mTOR (Ser2448) Recombinant antibody

Phospho-mTOR (Ser2448) Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat and More (4)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3L18

Cat no : 80596-1-RR

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1



Tested Applications

Positive WB detected inHEK-293 cells, Calyculin A treated HSC-T6 cells, HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated HeLa cells
Positive IF detected inPMA treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 16 publications below

Product Information

80596-1-RR targets Phospho-mTOR (Ser2448) in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated Molecular Weight 289 kDa
Observed Molecular Weight250-289 kDa
GenBank Accession NumberBC117166
Gene Symbol FRAP1
Gene ID (NCBI) 2475
RRIDAB_2918905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

Protocols

Product Specific Protocols
WB protocol for Phospho-mTOR (Ser2448) antibody 80596-1-RRDownload protocol
IF protocol for Phospho-mTOR (Ser2448) antibody 80596-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Affect Disord

Prenatal alcohol exposure enhanced alcohol preference and susceptibility to PTSD in a sex-dependent manner through the synaptic HCN1 channel

Authors - Hui Yao
humanWB

Front Cell Dev Biol

The Establishment of Quantitatively Regulating Expression Cassette with sgRNA Targeting BIRC5 to Elucidate the Synergistic Pathway of Survivin with P-Glycoprotein in Cancer Multi-Drug Resistance.

Authors - Changping Deng
humanWB

FASEB J

Activation of moderate autophagy promotes survival of fat graft

Authors - Su-Jun Pei
ratWB

J Ethnopharmacol

Anti-apoptotic effect of HeidihuangWan in renal tubular epithelial cells via PI3K/Akt/mTOR signaling pathway

Authors - Ying-Ying Li
humanWB

J Biochem Mol Toxicol

ATL1 inhibits the proliferation and invasion of trophoblast cells via inhibition of the mTOR signaling pathway

Authors - Guanli Zhang
humanWB

Int J Ophthalmol

Effects of Lycium barbarum polysaccharide on the photoinduced autophagy of retinal pigment epithelium cells.

Authors - Yuan-Yuan Gao