Piezo2 Polyclonal antibody

Piezo2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26205-1-AP

Synonyms

C18orf30, FAM38B, Piezo2, Transmembrane protein C18orf30

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Validation Data Gallery

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  IHC staining of human liver using 26205-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26205-1-AP (Piezo2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26205-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26205-1-AP (Piezo2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

26205-1-AP targets Piezo2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Piezo2 fusion protein Ag24528
• Full Name: family with sequence similarity 38, member B
• Calculated molecular weight: 318 kDa
• GenBank accession number: AB527139
• Gene symbol: FAM38B
• Gene ID (NCBI): 63895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM38B, also named as PIEZO2, is a mechanosensitive, rapidly inactivating (RI) ion channel which is open and converts the mechanical stimulus signals into bioelectrical signals after stimulated by mechanical signals. FAM38B has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. It plays an important role in the biological process, maintaining cell metabolism and cell migration. Loss-of-function mutations in the human FAM38B gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis.

Publications

Species	Application	Title
mouse	WB	Glia; Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes; Authors - Yue Wan
mouse	IHC	Int J Biol Sci; Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing.; Authors - Yunlu Liu
human,mouse	IHC	Adv Clin Exp Med; PIEZO2 promotes cell proliferation and metastasis in colon carcinoma through the SLIT2/ROBO1/VEGFC pathway; Authors - Haotian Shang
mouse	IHC	Cells; Midpalatal Suture: Single-Cell RNA-Seq Reveals Intramembrane Ossification and Piezo2 Chondrogenic Mesenchymal Cell Involvement; Authors - Lu Gao