FAM38B Polyclonal antibody

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  WB analysis using 26205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26205-1-AP (FAM38B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26205-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26205-1-AP (Piezo2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26205-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26205-1-AP (FAM38B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 26205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26205-1-AP (FAM38B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 26205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26205-1-AP (FAM38B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM38B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26205-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

Piezo2, Transmembrane protein C18orf30, C18orf30

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Tested Applications

• Positive WB detected in: HUVEC cells, Neuro-2a cells, SK-N-SH cells
• Positive IHC detected in: human liver tissue, mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

26205-1-AP targets FAM38B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FAM38B fusion protein Ag24528
• Full Name: family with sequence similarity 38, member B
• Calculated Molecular Weight: 318 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: AB527139
• Gene Symbol: FAM38B
• Gene ID (NCBI): 63895
• RRID: AB_2880425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H5I5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FAM38B, also named as PIEZO2, is a mechanosensitive, rapidly inactivating (RI) ion channel which is open and converts the mechanical stimulus signals into bioelectrical signals after stimulated by mechanical signals. FAM38B has been recently identified in dorsal root ganglion (DRG) neurons to mediate tactile transduction. It plays an important role in the biological process, maintaining cell metabolism and cell migration. Loss-of-function mutations in the human FAM38B gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis.The 80 kDa band detected by SDS-PAGE can be caused by alternative splicing (PMID: 34335288, 37227654).

Publications

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); The Critical Role of The Piezo1/β-catenin/ATF4 Axis on The Stemness of Gli1+ BMSCs During Simulated Microgravity-Induced Bone Loss; Authors - Yuxiang Hu
mouse	WB	Glia; Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes; Authors - Yue Wan
mouse	IHC	Int J Biol Sci; Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing.; Authors - Yunlu Liu
human,mouse	IHC	Adv Clin Exp Med; PIEZO2 promotes cell proliferation and metastasis in colon carcinoma through the SLIT2/ROBO1/VEGFC pathway; Authors - Haotian Shang
mouse	IHC	Cells; Midpalatal Suture: Single-Cell RNA-Seq Reveals Intramembrane Ossification and Piezo2 Chondrogenic Mesenchymal Cell Involvement; Authors - Lu Gao
mouse	IF	Front Mol Neurosci; Activation of mechanoreceptor Piezo1 inhibits enteric neuronal growth and migration in vitro; Authors - Chioma Moneme