Podocalyxin Recombinant monoclonal antibody

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• Podocalyxin Recombinant monoclonal antibody (87582-2-RR)

• 

  WB analysis using 87582-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87582-2-RR (Podocalyxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87582-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87582-2-RR (Podocalyxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 87582-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 87582-2-RR (Podocalyxin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 87582-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 87582-2-RR (Podocalyxin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 87582-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Podocalyxin antibody (87582-2-RR, Clone: 252967A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 87582-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Podocalyxin antibody (87582-2-RR, Clone: 252967A3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 87582-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Podocalyxin antibody (87582-2-RR, Clone: 252967A3 ) at dilution of 1:500 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9), CoraLite®594 ACE2 antibody (CL594-21115, red), E-cadherin antibody (60335-1-Ig, Clone: 6B11F11, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 87582-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Podocalyxin antibody (87582-2-RR, Clone: 252967A3 ) at dilution of 1:500 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L) (SA00014-9), CoraLite®594 ACE2 antibody (CL594-21115, red), E-cadherin antibody (60335-1-Ig, Clone: 6B11F11, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity of 87582-2-RR

  Biolayer interferometry (BLl) kinetic assays of 87582-2-RR against Mouse Podocalyxin were performed. The affinity constant is 0.548 nM.

Podocalyxin Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 87582-2-RR

Clone No.252967A3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

PC, Pclp1, PCLP-1, Podocalyxin-like protein 1, Podxl

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Tested Applications

• Positive WB detected in: mouse kidney tissue, bEnd.3 cells, 4T1 cells, mouse lung tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87582-2-RR targets Podocalyxin in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg7526

  Product name: Recombinant mouse Podocalyxin protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 22-404 aa of NM_013723.3

  Sequence: HNGNETSTSAIKSSTVQSHQSATTSTEVTTGHPVASTLASTQPSNPTPFTTSTQSPSMPTSTPNPTSNQSGGNLTSSVSEVDKTKTSSPSSTAFTSSSGQTASSGGKSGDSFTTAPTTTLGLINVSSQPTDLNTTSKLLSTPTTDNTTSPQQPVDSSPSTASHPVGQHTPAAVPSSSGSTPSTDNSTLTWKPTTHKPLGTSEATQPLTSQTPGITTLPVSTLQQSMASTVGTTTEEFTHLISNGTPVAPPGPSTPSPIWAFGNYQLNCEPPIRPDEELLILNLTRASLCERSPLDEKEKLVELLCHSVKASFKPAEDLCTLHVAPILDNQAVAVKRIIIETKLSPKAVYELLKDRWDDLTEAGVSDMKLGKEGPPEVNEDRFS

• Full Name: podocalyxin-like
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 140-160 kDa
• GenBank Accession Number: NM_013723.3
• Gene Symbol: Podxl
• Gene ID (NCBI): 27205
• RRID: AB_3745635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9R0M4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It is involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).