Podoplanin Monoclonal antibody, PBS Only

Podoplanin Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D9F3

Cat no : 67432-1-PBS

Synonyms

AGGRUS, D2 40, D2 40,M2A, D240, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, podoplanin, PSEC0025, T1 alpha, T1A, T1A 2

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Validation Data Gallery

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  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (PDPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67432-1-PBS targets Podoplanin in IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Podoplanin fusion protein Ag17691
• Full Name: podoplanin
• Calculated Molecular Weight: 238 aa, 25 kDa
• GenBank Accession Number: BC022812
• Gene Symbol: PDPN
• Gene ID (NCBI): 10630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Podoplanin was identified as a glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.