Anti-Mouse Podoplanin Rabbit Recombinant Antibody

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  FC experiment of C2C12 using 98492-3-RR

  1x10^6 C2C12 cells were surface stained with 0.25 ug Anti-Mouse Podoplanin Rabbit RecAb (98492-3-RR, Clone: 250618A2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Podoplanin Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98492-3-RR

Clone No.250618A2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

E11, Glycoprotein 38, Gp38, Ots8, OTS-8

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Tested Applications

• Positive FC detected in: C2C12 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98492-3-RR targets Podoplanin in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Podoplanin fusion protein Eg2861
• Full Name: podoplanin
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_010329.3
• Gene Symbol: Pdpn
• Gene ID (NCBI): 14726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62011
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Podoplanin was identified as a glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, Podoplanin induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. Podoplanin induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.