Polycystin 1 Polyclonal antibody

Polycystin 1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 22263-1-AP

Synonyms

PBP, PC1, PKD1, Polycystin 1

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Validation Data Gallery

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  IHC staining of human liver using 22263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22263-1-AP (Polycystin 1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 22263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22263-1-AP (Polycystin 1 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 22263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22263-1-AP (Polycystin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive IHC detected in: human liver tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22263-1-AP targets Polycystin 1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polycystic kidney disease 1 (autosomal dominant)
• Calculated Molecular Weight: 463 kDa
• GenBank Accession Number: NM_000296
• Gene Symbol: PKD1
• Gene ID (NCBI): 5310
• RRID: AB_2879053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polycystin 1 (PC1) is also named as PKD1, and Autosomal dominant polycystic kidney disease 1 protein, and belongs to the polycystin family. PKD1 and PKD2 are formed component of a heteromeric calcium-permeable ion channel. PKD1 is considered an orphan, atypical G protein coupled receptor complexed with TRPP2 (Polycystin 2 or PKD2), a Ca2+-permeable ion channel (PMID:27214281). PKD1 is involved in renal tubulogenesis (PMID:12482949).