Presenilin-1-Specific Recombinant monoclonal antibody

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  WB analysis using 83618-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 83618-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Presenilin-1-Specific antibody (83618-5-RR, Clone: 250834G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Presenilin-1-Specific Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83618-5-RR

Clone No.250834G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

Presenilin-1, PSEN1, AD3, presenilin 1, Presenilin1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, Caco-2 cells, COS-7 cells, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83618-5-RR targets Presenilin-1-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: presenilin 1
• Calculated Molecular Weight: 467 aa, 53 kDa
• Observed Molecular Weight: 21 kDa, 53 kDa
• GenBank Accession Number: BC011729
• Gene Symbol: PSEN1
• Gene ID (NCBI): 5663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49768
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.