Various lysates were subjected to SDS PAGE followed by western blot with 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of Human brain cancer（Astrocitoma） using 83618-5-RR
Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 83618-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Jurkat using 83618-5-RR
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Presenilin-1-Specific antibody (83618-5-RR, Clone: 250834G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Presenilin-1-Specific antibody (83618-5-RR, Clone: 250834G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
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HeLa cells, HepG2 cells, Jurkat cells, Caco-2 cells, COS-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected in
mouse brain tissue, Human brain cancer（Astrocitoma） Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Jurkat cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
83618-5-RR targets Presenilin-1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.
Protocols
Product Specific Protocols
IF protocol for Presenilin-1-Specific antibody 83618-5-RR
Various lysates were subjected to SDS PAGE followed by western blot with 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of Human brain cancer（Astrocitoma） using 83618-5-RR
Immunohistochemical analysis of paraffin-embedded Human brain cancer(Astrocitoma) slide using 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 83618-5-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-5-RR (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of Jurkat using 83618-5-RR
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Presenilin-1-Specific antibody (83618-5-RR, Clone: 250834G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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