Prohibitin 2 Monoclonal antibody, PBS Only

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  WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

  WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin 2 antibody (66424-1-Ig, Clone: 1D9C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Prohibitin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66424-1-PBS

Clone No.1D9C7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PHB2, 1D9C7, BAP, B-cell receptor-associated protein BAP37, D prohibitin

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Product Information

66424-1-PBS targets Prohibitin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Prohibitin 2 fusion protein Ag2977
• Full Name: prohibitin 2
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC014766
• Gene Symbol: Prohibitin 2
• Gene ID (NCBI): 11331
• RRID: AB_2811041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q99623
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)