Mouse Psap Recombinant monoclonal antibody, PBS Only (Capture/Detector)

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  Sandwich ELISA standard curve of MP02256-2

  Sandwich ELISA standard curve of MP02256-2, Mouse Psap Recombinant Matched Antibody Pair - PBS only. 86043-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3155. 86043-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP02256-1

  The mean Psap concentration was determined to be 491.7 ng/mL in mouse serum.

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  Sample test of MP02256-2

  Serum of sixteen mice was measured. The Psap concentration of detected samples was determined to be 396.8 ng/mL with a range of 2,614.3-9,786.9 ng/mL.

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  Cytometric bead array standard curve of MP02256-1

  Cytometric bead array standard curve of MP02256-1, MOUSE Psap Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86043-2-PBS. Detection antibody: 86043-1-PBS. Standard: Eg3155. Range: 0.313-40 ng/mL.

Psap Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86043-1-PBS

Clone No.250312G2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Prosaposin, Saposin-A, Saposin-B, Saposin-B-Val, Saposin-C

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Product Information

86043-1-PBS targets Psap as part of a matched antibody pair:

MP02256-1: 86043-2-PBS capture and 86043-1-PBS detection (validated in Cytometric bead array)

MP02256-2: 86043-1-PBS capture and 86043-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3155

  Product name: Recombinant Mouse PSAP/Prosaposin protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 17-557 aa of NM_011179.3

  Sequence: SPVQDPKTCSGGSAVLCRDVKTAVDCGAVKHCQQMVWSKPTAKSLPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDMIKGEMSNPGEVCSALNLCQSLQEYLAEQNQKQLESNKIPEVDMARVVAPFMSNIPLLLYPQDHPRSQPQPKANEDVCQDCMKLVSDVQTAVKTNSSFIQGFVDHVKEDCDRLGPGVSDICKNYVDQYSEVCVQMLMHMQDQQPKEICVLAGFCNEVKRVPMKTLVPATETIKNILPALEMMDPYEQNLVQAHNVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLDIFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAHVPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGTEKCVWGPSYWCQNMETAARCNAVDHCKRHVWN

• Full Name: prosaposin
• Calculated Molecular Weight: 61 kDa
• GenBank Accession Number: NM_011179.3
• Gene Symbol: Psap
• Gene ID (NCBI): 19156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: NP_035309.3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.