QARS Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86333-3-RR (same clone as 86333-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86333-3-RR (QARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86333-3-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 86333-3-RR (same clone as 86333-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using QARS antibody (86333-3-RR, Clone: 251052A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86333-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86333-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86333-3-RR against Human QARS were performed. The affinity constant is 0.176 nM.

QARS Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86333-3-PBS

Clone No.251052A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GlnRS, EC:6.1.1.18, Glutamine--tRNA ligase, Glutaminyl-tRNA synthetase, QARS1

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Product Information

86333-3-PBS targets QARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3375

  Product name: Recombinant human QARS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 362-717 aa of BC016634

  Sequence: VKYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALDVYCPVQWEYGRLNLHYAVVSKRKILQLVATGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDVLNDTAPRAMAVLESLRVIITNFPAAKSLDIQVPNFPADETKGFHQVPFAPIVFIERTDFKEEPEPGFKRLAWGQPVGLRHTGYVIELQHVVKGPSGCVESLEVTCRRADAGEKPKAFIHWVSQPLMCEVRLYERLFQHKNPEDPTEVPGGFLSDLNLASLHVVDAALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGKLVFNRTVTLKEDPGKV

• Full Name: glutaminyl-tRNA synthetase
• Calculated Molecular Weight: 775 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC016634
• Gene Symbol: GlnRS
• Gene ID (NCBI): 5859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47897
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).