QKI Polyclonal antibody

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• KD/KO Validated

QKI Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 13169-1-AP

Synonyms

QK, Protein quaking, KH domain-containing RNA-binding protein QKI, HqkI, Hqk

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Validation Data Gallery

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  WB analysis using 13169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13169-1-AP (QKI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13169-1-AP

  IP result of anti-QKI (IP:13169-1-AP, 4ug; Detection:13169-1-AP 1:400) with K-562 cells lysate 3600ug.

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  IHC staining of human stomach cancer using 13169-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13169-1-AP (QKI antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, PC-12 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13169-1-AP targets QKI in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: QKI fusion protein Ag3822
• Full Name: quaking homolog, KH domain RNA binding (mouse)
• Calculated Molecular Weight: 341 aa, 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC019917
• Gene Symbol: QKI
• Gene ID (NCBI): 9444
• RRID: AB_2173138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

QKI (Quaking) is RNA-binding protein that plays a central role in myelinization (PMID: 16641098). It regulates pre-mRNA splicing, mRNA export and protein translation (PMID: 16641098). It acts upstream of or within several processes, including long-chain fatty acid biosynthetic process, positive regulation of macromolecule metabolic process and vasculogenesis. QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism. It is expressed in the frontal cortex of brain (PMID: 16342280).

Publications

Species	Application	Title
human	WB	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
human	WB,IHC	Cell Oncol (Dordr); QKI deficiency maintains glioma stem cell stemness by activating the SHH/GLI1 signaling pathway.; Authors - Bo Han
	WB	Oxid Med Cell Longev; MicroRNA-155 Promotes Myocardial Infarction-Induced Apoptosis by Targeting RNA-Binding Protein QKI.; Authors - Jing Guo
human	WB	J Biol Chem; MicroRNA-214 Promotes Dendritic Development by Targeting the Schizophrenia-associated Gene Quaking.; Authors - Koichiro Irie ; KD Validated
human	WB	J Biol Chem; RNA binding protein Quaking stabilizes Sirt2 mRNA during oligodendroglial differentiation.; Authors - Merlin P Thangaraj
human,mouse	WB,IHC,IF	J Cell Physiol; Quaking promotes the odontoblastic differentiation of human dental pulp stem cells.; Authors - Shuchen Li ; KD Validated