• Featured Product
  • KD/KO Validated

QPRT Polyclonal antibody

QPRT Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25174-1-AP

Synonyms

QAPRTase, QPRT, QPRTase



Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25174-1-AP targets QPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen QPRT fusion protein Ag17680
Full Name quinolinate phosphoribosyltransferase
Calculated Molecular Weight 297 aa, 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC018910
Gene Symbol QPRT
Gene ID (NCBI) 23475
RRIDAB_2879941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.

Protocols

Product Specific Protocols
WB protocol for QPRT antibody 25174-1-APDownload protocol
IF protocol for QPRT antibody 25174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney.

Authors - Xiao Y Zhou
human

J Immunol

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues

Authors - Teresa W-M Fan
humanWB

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin
mouseIF

Aging Cell

Deletion of enzymes for de novo NAD+ biosynthesis accelerated ovarian aging

Authors - Qingling Yang
human,mouseWB

Nat Commun

Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas

Authors - Jianxing Yin
  • KD Validated
mouseWB,IHC,IF

Front Endocrinol (Lausanne)

Unraveling the nexus of NAD+ metabolism and diabetic kidney disease: insights from murine models and human data

Authors - Sisi Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kus (Verified Customer) (12-08-2022)

Antibody works for WB in 1:500 or 1;1000 dilution

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Liver cells
QPRT Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:25174-1-AP)