QPRT Polyclonal antibody

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  WB analysis using 25174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25174-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 25174-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 25174-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using QPRT antibody (25174-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

QPRT Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25174-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.4.2.19, Nicotinate-nucleotide pyrophosphorylase [carboxylating], QAPRTase, QPRTase

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

25174-1-AP targets QPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17680

  Product name: Recombinant human QPRT protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-297 aa of BC018910

  Sequence: MDAEGLALLLPPVTLAALVDSWLREDCPGLNYAALVSGAGPSQAALWAKSPGVLAGQPFFDAIFTQLNCQVSWFLPEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKIH

• Full Name: quinolinate phosphoribosyltransferase
• Calculated Molecular Weight: 297 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC018910
• Gene Symbol: QPRT
• Gene ID (NCBI): 23475
• RRID: AB_2879941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15274
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Front Genet; Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney.; Authors - Xiao Y Zhou
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan
human	WB	J Biol Chem; Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity; Authors - Xiaohan Jin
mouse	IF	Aging Cell; Deletion of enzymes for de novo NAD+ biosynthesis accelerated ovarian aging; Authors - Qingling Yang
mouse,human	WB	Nat Commun; Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas; Authors - Jianxing Yin ; KD Validated
mouse	WB,IHC,IF	Front Endocrinol (Lausanne); Unraveling the nexus of NAD+ metabolism and diabetic kidney disease: insights from murine models and human data; Authors - Sisi Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kus (Verified Customer) (12-08-2022)

Antibody works for WB in 1:500 or 1;1000 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

QPRT Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:25174-1-AP)