Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25174-1-AP targets QPRT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17680 Product name: Recombinant human QPRT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-297 aa of BC018910 Sequence: MDAEGLALLLPPVTLAALVDSWLREDCPGLNYAALVSGAGPSQAALWAKSPGVLAGQPFFDAIFTQLNCQVSWFLPEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKIH Predict reactive species |
| Full Name | quinolinate phosphoribosyltransferase |
| Calculated Molecular Weight | 297 aa, 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC018910 |
| Gene Symbol | QPRT |
| Gene ID (NCBI) | 23475 |
| RRID | AB_2879941 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15274 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for QPRT antibody 25174-1-AP | Download protocol |
| WB protocol for QPRT antibody 25174-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
QPRT Polyclonal antibody (Cat# 25174-1-AP) has 15 citations across journals including Nature Communications, Aging Cell, Journal of Biological Chemistry, Journal of Immunology, Science of the Total Environment, British Journal of Pharmacology, Breast Cancer Research, Frontiers in Endocrinology, Bioorganic Chemistry, Cancer Research Communications, Frontiers in Genetics, medRxiv, Frontiers in Pharmacology, and Intensive Care Medicine Experimental. Research fields span NAD⁺ metabolism, glioblastoma, aging, IBD/ulcerative colitis, breast cancer, muscle atrophy, kidney disease, and neuroinflammation.
| Species | Application | Title |
|---|---|---|
Nat Commun Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas
| ||
Sci Total Environ 3-hydroxykynurenine increase in kynurenine pathway driven bisphenol F induced anxiety- and depression-like behaviors. | ||
Aging Cell Nicotinamide Riboside Supplementation Alleviates Testicular Aging Induced by Disruption of Qprt-Dependent NAD+ De Novo Synthesis in Mice
| ||
Br J Pharmacol Tryptophan 2,3-dioxygenase deficiency attenuates dextran sodium sulphate -induced ulcerative colitis in mice by suppressing macrophage polarisation and reducing intestinal epithelial cell apoptosis. | ||
Breast Cancer Res Integrated multi-omics analysis and functional validation reveal the role of TRIM2 as a potential novel biomarker in breast cancer. |
Reviews
What customers say
The single review for this product rates it 3 out of 5 stars. The reviewer used the antibody for Western Blot on human liver cells at a 1:1000 dilution and noted that it works well at both 1:500 and 1:1000 dilutions. Overall, the feedback is positive regarding performance in WB, though the moderate rating suggests there may be room for improvement in other aspects.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kus (Verified Customer) (12-08-2022) | Antibody works for WB in 1:500 or 1;1000 dilution
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