QTRT1 Polyclonal antibody

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  WB analysis of HeLa using 12349-1-AP

  WB result of QTRT1 antibody (12349-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-QTRT1 transfected HeLa cells.

• Featured Product
• KD/KO Validated

QTRT1 Polyclonal Antibody for WB, ELISA

Cat No. 12349-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.4.2.64, FP3235, Guanine insertion enzyme, Queuine tRNA-ribosyltransferase catalytic subunit 1, TGT

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12349-1-AP targets QTRT1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: QTRT1 fusion protein Ag3010
• Full Name: queuine tRNA-ribosyltransferase 1
• Calculated Molecular Weight: 403 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC015350
• Gene Symbol: QTRT1
• Gene ID (NCBI): 81890
• RRID: AB_2877847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BXR0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

QTRT1 (Queuine tRNA-ribosyltransferase catalytic subunit 1), also known as tRNA-guanine transglycosylase (TGT), is a key enzymeinvolved in the post-transcriptional modification of tRNAs. In humans, the QTRT1 and QTRT2 heterodimer complex exhibits transglycosylase activity, and replaces the guanine base of the first letter of the tRNA anticodon with q, thus forming Q on the tRNA (PMID: 30149595, 25884661). High QTRT1 expression is a biomarkerfor poor prognosis in LUAD patients, and QTRT1 mayfunction as a latent tumor biomarker in the prognosticprediction for LUAD (PMID: 33490170).