RAB11B Polyclonal antibody

RAB11B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28498-1-AP

Synonyms

GTP binding protein YPT3, H YPT3, RAB11B, Ras related protein Rab 11B, YPT3

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Validation Data Gallery

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  WB analysis using 28498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28498-1-AP (RAB11B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28498-1-AP (RAB11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 28498-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11B antibody (28498-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, PC-12 cells, SH-SY5Y cells, RAW 264.7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue, NIH3T3 cells
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28498-1-AP targets RAB11B in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB11B fusion protein Ag25429
• Full Name: RAB11B, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC110081
• Gene Symbol: RAB11B
• Gene ID (NCBI): 9230
• RRID: AB_2881156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Pathol Res Pract; SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma; Authors - Chuandong Cheng