RAB11FIP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB11FIP5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14594-1-AP

Synonyms

Gaf 1, GAF1, Gamma SNAP associated factor 1, KIAA0857, Phosphoprotein pp75, pp75, Rab11 FIP5, RAB11FIP5, RIP11

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Validation Data Gallery

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14594-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14594-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14594-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14594-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, mouse lung tissue, human heart tissue, mouse kidney tissue, HeLa cells, mouse heart tissue, HEK-293T cells
• Positive IHC detected in: human cervical cancer tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

14594-1-AP targets RAB11FIP5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, mouse, rat samples.

• Tested Reactivity: human, mouse, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB11FIP5 fusion protein Ag6128
• Full Name: RAB11 family interacting protein 5 (class I)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC035013
• Gene Symbol: RAB11FIP5
• Gene ID (NCBI): 26056
• RRID: AB_10860101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab11 family-interacting protein 5 (Rab11fip5) is an adaptor protein that binds to the small GTPase Rab11, which has an important function in endosome recycling and trafficking of cellular proteins to the plasma membrane. Rab11fip5 is involved in many cellular processes, such as cytoskeleton rearrangement, iron uptake and exocytosis in neuroendocrine cells, and is also known as a candidate gene for autism-spectrum disorder.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.; Authors - Kai He ; KD Validated