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  • KD/KO Validated

RAB12 Polyclonal antibody

RAB12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18843-1-AP

Synonyms

RAB12, Ras related protein Rab 12



Tested Applications

Positive WB detected infetal human brain tissue, HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18843-1-AP targets RAB12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RAB12, member RAS oncogene family
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberNM_001025300
Gene Symbol RAB12
Gene ID (NCBI) 201475
RRIDAB_10603469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.

Protocols

Product Specific Protocols
WB protocol for RAB12 antibody 18843-1-APDownload protocol
IHC protocol for RAB12 antibody 18843-1-APDownload protocol
IF protocol for RAB12 antibody 18843-1-APDownload protocol
IP protocol for RAB12 antibody 18843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Biol

Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes.

Authors - C Alexander Boecker
humanWB

EMBO Rep

Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy.

Authors - Jie Xu
humanWB

Neurobiol Dis

Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment.

Authors - Jillian H Kluss
mouseWB

J Immunol

Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.

Authors - Adi Efergan
  • KD Validated
humanWB

Front Oncol

Rab12 Promotes Radioresistance of HPV-Positive Cervical Cancer Cells by Increasing G2/M Arrest.

Authors - Yujie Huang
  • KD Validated
humanWB

Traffic

Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.

Authors - Gustaf E Rydell
  • KD Validated