Tested Applications
| Positive WB detected in | fetal human brain tissue, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18843-1-AP targets RAB12 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | RAB12, member RAS oncogene family |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | NM_001025300 |
| Gene Symbol | RAB12 |
| Gene ID (NCBI) | 201475 |
| RRID | AB_10603469 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IQ22 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB12 antibody 18843-1-AP | Download protocol |
| IHC protocol for RAB12 antibody 18843-1-AP | Download protocol |
| IP protocol for RAB12 antibody 18843-1-AP | Download protocol |
| WB protocol for RAB12 antibody 18843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB12 Polyclonal antibody (Cat# 18843-1-AP) has 32 citations published in journals including NPJ Parkinson's Disease, Molecular Psychiatry, Acta Neuropathologica, Current Biology, eLife, EMBO Reports, Science Immunology, Molecular Neurodegeneration, Neurobiology of Disease, Journal of Immunology, Biochemical Journal, and others. Associated research fields include Parkinson's disease, neurodegeneration, autophagy, mitophagy, lysosomal biology, LRRK2–Rab12 signaling, neuroinflammation, cancer, osteoporosis, and retinal degeneration.
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Inflammatory signaling differentially changes chromatin accessibility and gene expression of the PD- associated kinase LRRK2 between human and mice. | ||
Sci Immunol Macrophage LRRK2 hyperactivity impairs autophagy and induces Paneth cell dysfunction | ||
Mol Psychiatry LRRK2 mediates haloperidol-induced changes in indirect pathway striatal projection neurons | ||
Acta Neuropathol LRRK2 kinase-mediated accumulation of lysosome-associated phospho-Rabs in tauopathies and synucleinopathies. | ||
NPJ Parkinsons Dis Adult-onset deletion of ATP13A2 in mice induces progressive nigrostriatal pathway dopaminergic degeneration and lysosomal abnormalities | ||
NPJ Parkinsons Dis Emergent glutamate & dopamine dysfunction in VPS35(D620N) knock-in mice and rapid reversal by LRRK2 inhibition |
