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  • KD/KO Validated

RAB14 Polyclonal antibody

RAB14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15662-1-AP

Synonyms

FBP, RAB 14, RAB14, Ras related protein Rab 14



Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, HepG2 cells, Raji cells, human brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15662-1-AP targets RAB14 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB14 fusion protein Ag8092
Full Name RAB14, member RAS oncogene family
Calculated Molecular Weight 215 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC006081
Gene Symbol RAB14
Gene ID (NCBI) 51552
RRIDAB_2173630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB14 antibody 15662-1-APDownload protocol
IHC protocol for RAB14 antibody 15662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Physiol Biochem

Hypermethylation of miR-338-3p and Impact of its Suppression on Cell Metastasis Through N-Cadherin Accumulation at the Cell -Cell Junction and Degradation of MMP in Gastric Cancer.

Authors - Bo Guo
humanIHC,WB

Onco Targets Ther

Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers.

Authors - Jun Zhang
humanWB

PLoS One

Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway.

Authors - Bo Guo
humanWB,IHC

Virchows Arch

PMID: 30267303

Authors - Jinnian Ge
  • KD Validated
humanWB,IHC

Tumour Biol

Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway.

Authors - Rui Hou
  • KD Validated
humanWB

Eur Rev Med Pharmacol Sci

STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14.

Authors - C Du