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RAB17 Polyclonal antibody
RAB17 Polyclonal Antibody for IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17501-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | WIF-B cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:25-1:50 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
17501-1-AP targets RAB17 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RAB17 fusion protein Ag11688 |
| Full Name | RAB17, member RAS oncogene family |
| Calculated Molecular Weight | 212 aa, 23 kDa |
| GenBank Accession Number | BC050426 |
| Gene Symbol | RAB17 |
| Gene ID (NCBI) | 64284 |
| RRID | AB_2878413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB17 is a member of the RAS oncogene family and is classified as a small GTPase that plays a crucial role in intracellular membrane trafficking It is specifically expressed in epithelial cells and is involved in various cellular functions such as transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. This process is mainly observed in epithelial cells and is important for the transcellular transport of substances like immunoglobulins from the basolateral surface to the apical surface. RAB17 is also implicated in the regulation of melanosome transport and release from melanocytes, which is critical for pigmentation in skin cells . Additionally, it has been suggested to regulate dendrite and dendritic spine development, which are important for neuronal function. The protein is involved in membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAB17 antibody 17501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer.
| ||
Cell Death Dis RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis
| ||
Int J Mol Sci The Tolerogenic Influence of Dexamethasone on Dendritic Cells Is Accompanied by the Induction of Efferocytosis, Promoted by MERTK | ||
Cell Death Dis Rab17 mediates differential antigen sorting following efferocytosis and phagocytosis. | ||
J Biol Chem The GTP-bound and Sumoylated Form of the rab17 Small Molecular Weight GTPase Selectively Binds Syntaxin 2 in Polarized Hepatic WIF-B Cells. | ||
