Tested Applications
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | WIF-B cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:25-1:50 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17501-1-AP targets RAB17 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11688 Product name: Recombinant human RAB17 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-212 aa of BC050426 Sequence: MAQAHRTPQPRAAPSQPRVFKLVLLGSGSVGKSSLALRYVKNDFKSILPTVGCAFFTKVVDVGATSLKLEIWDTAGQEKYHSVCHLYFRGANAALLVYDITRKDSFLKAQQWLKDLEEELHPGEVLVMLVGNKTDLSQEREVTFQEGKEFADSQKLLFMETSAKLNHQVSEVFNTVAQELLQRSDEEGQALRGDAAVALNKGPARQAKCCAH Predict reactive species |
| Full Name | RAB17, member RAS oncogene family |
| Calculated Molecular Weight | 212 aa, 23 kDa |
| GenBank Accession Number | BC050426 |
| Gene Symbol | RAB17 |
| Gene ID (NCBI) | 64284 |
| RRID | AB_2878413 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0T7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB17 is a member of the RAS oncogene family and is classified as a small GTPase that plays a crucial role in intracellular membrane trafficking It is specifically expressed in epithelial cells and is involved in various cellular functions such as transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. This process is mainly observed in epithelial cells and is important for the transcellular transport of substances like immunoglobulins from the basolateral surface to the apical surface. RAB17 is also implicated in the regulation of melanosome transport and release from melanocytes, which is critical for pigmentation in skin cells . Additionally, it has been suggested to regulate dendrite and dendritic spine development, which are important for neuronal function. The protein is involved in membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAB17 antibody 17501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB17 Polyclonal antibody (Cat# 17501-1-AP) has 10 citations published in journals including Nature Communications, Molecular Cancer, Cell Death & Disease, Journal of Biological Chemistry, Cell Signaling, International Journal of Molecular Sciences, International Immunopharmacology, Frontiers in Genetics, and Molecular Biology of the Cell. Research fields span cancer biology (lung, endometrial, renal, and colorectal cancers), intracellular membrane trafficking, efferocytosis, ferroptosis, viral protein transport, and immunotherapy response prediction.
| Species | Application | Title |
|---|---|---|
Mol Cancer Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer.
| ||
Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane
| ||
Cell Death Dis Rab17 mediates differential antigen sorting following efferocytosis and phagocytosis. | ||
Cell Death Dis RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis
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Int Immunopharmacol ACOT8-mediated palmitate accumulation promotes M1 macrophage polarization in renal ischemia-reperfusion injury via activation of the cGAS-STING pathway. | ||
Int J Mol Sci The Tolerogenic Influence of Dexamethasone on Dendritic Cells Is Accompanied by the Induction of Efferocytosis, Promoted by MERTK |
