Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, HEK-293 cells, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11101-1-AP targets Rab23 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1588 Product name: Recombinant human RAB23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-203 aa of BC015021 Sequence: MLEEDMEVAIKMVVVGNGAVGKSSMIQRYCKGIFTKDYKKTIGVDFLERQIQVNDEDVRLMLWDTAGQEEFDAITKAYYRGAQACVLVFSTTDRESFEAVSSWREKVVAEVGDIPTVLVQNKIDLLDDSCIKNEEAEALAKRLKLRFYRTSVKEDLNVNEVFKYLAEKYLQKLKQQIAEDPELTHSSSNKIGVFNTSGGSHSG Predict reactive species |
| Full Name | RAB23, member RAS oncogene family |
| Calculated Molecular Weight | 237 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC015021 |
| Gene Symbol | RAB23 |
| Gene ID (NCBI) | 51715 |
| RRID | AB_2173784 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULC3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Rab23 antibody 11101-1-AP | Download protocol |
| IP protocol for Rab23 antibody 11101-1-AP | Download protocol |
| WB protocol for Rab23 antibody 11101-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
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| Species | Application | Title |
|---|---|---|
Nat Commun Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane
| ||
Curr Biol Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.
| ||
Cell Mol Life Sci RAB23 facilitates clathrin-coated nascent vesicle formation at the plasma membrane and modulates cell signaling | ||
Oncotarget Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.
| ||
Cells Cap-Specific m6Am Methyltransferase PCIF1/CAPAM Regulates mRNA Stability of RAB23 and CNOT6 through the m6A Methyltransferase Activity | ||
Reviews
What customers say
The single review for 11101-1-AP (rated 3/5) reports use in Western Blot at a 1:500 dilution on ARPE-19 cells. The reviewer observed multiple bands distributed at approximately 50 and 75 kDa, and noted that this result may not reflect performance in other sample types. Overall, the feedback suggests the antibody produced non-specific banding in this particular application and cell line.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Guorong (Verified Customer) (11-22-2022) | Multiple bands distributed at 50 and 75 kDa were observed . Does not represent the performance in other kind of samples
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