Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 11445-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11445-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11445-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11445-1-AP
IP result of anti-RAB24 (IP:11445-1-AP, 4ug; Detection:11445-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.
IP result of anti-RAB24 (IP:11445-1-AP, 4ug; Detection:11445-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.
IHC staining of human gliomas using 11445-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11445-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HEK-293 cells, human skeletal muscle tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected in
mouse brain tissue
Positive IHC detected in
human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking. Unlike other Rab family members, RAB24 does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB. It Interacts with ZFYVE20. Rab24 modulates several mitotic events, including chromosome segregation and cytokinesis, perhaps through the interaction with microtubules.
Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 11445-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 11445-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11445-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human gliomas using 11445-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11445-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse brain using 11445-1-AP
IP result of anti-RAB24 (IP:11445-1-AP, 4ug; Detection:11445-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RAB24 Polyclonal antibody
Catalog Number
11445-1-AP
Citations
2
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500
Applications
WB, IP, IHC, ELISA
Reactivity
human, mouse, rat, canine
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
