RAB24 Polyclonal antibody

RAB24 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11445-1-AP

Synonyms

RAB24, Ras related protein Rab 24

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Validation Data Gallery

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  WB analysis using 11445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11445-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11445-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11445-1-AP (RAB24 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11445-1-AP

  IP result of anti-RAB24 (IP:11445-1-AP, 4ug; Detection:11445-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of human gliomas using 11445-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11445-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11445-1-AP (RAB24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human skeletal muscle tissue, fetal human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11445-1-AP targets RAB24 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB24 fusion protein Ag1995
• Full Name: RAB24, member RAS oncogene family
• Calculated Molecular Weight: 203 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC021263
• Gene Symbol: RAB24
• Gene ID (NCBI): 53917
• RRID: AB_2269046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB24 is a small GTPase of the Rab subfamily of Ras-related proteins that regulate intracellular protein trafficking. Unlike other Rab family members, RAB24 does not interact with GDP dissociation inhibitors (GDIs), including ARHGDIA and ARHGDIB. It Interacts with ZFYVE20. Rab24 modulates several mitotic events, including chromosome segregation and cytokinesis, perhaps through the interaction with microtubules.

Publications

Species	Application	Title
canine	IHC	PLoS Genet; Canine Hereditary Ataxia in Old English Sheepdogs and Gordon Setters Is Associated with a Defect in the Autophagy Gene Encoding RAB24.; Authors - Caryline Agler
human	WB	Dose Response; Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.; Authors - Jianfa Yu