RAB25 Polyclonal antibody

• 

  WB analysis of HT-29 using 13189-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 13189-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 13189-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RAB25 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13189-1-AP

Publications(3)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CATX 8, CATX8, CATX-8, Ras related protein Rab 25, Ras-related protein Rab-25

---

Tested Applications

• Positive WB detected in: HT-29 cells, Caco-2 cells, T-47D cells
• Positive IHC detected in: human renal cell carcinoma tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3864

  Product name: Recombinant human RAB25 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-213 aa of BC033322

  Sequence: MGNGTEEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAGLERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEARMFAENNGLLFLETSALDSTNVELAFETVLKEIFAKVSKQRQNSIRTNAITLGSAQAGQEPGPGEKRACCISL

• Full Name: RAB25, member RAS oncogene family
• Calculated Molecular Weight: 213 aa, 23 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC033322
• Gene Symbol: RAB25
• Gene ID (NCBI): 57111
• RRID: AB_2877921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P57735
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB25 (RAB25, member RAS oncogene family), also known as RAB11C or CATX-8, is a small GTPase belonging to the RAS superfamily of proteins. RAB25 is a key regulator of receptor recycling and polarized vesicular trafficking in epithelial cells. Unlike many RAB proteins, RAB25 expression is predominantly restricted to epithelial tissues and is frequently dysregulated in carcinomas. It functions as a critical determinant of cellular fate through its context-dependent roles in modulating major signaling pathways, including integrin recycling, growth factor receptor trafficking (e.g., EGFR, IGF1R), and autophagy.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	FEBS J; The protease ADAMTS5 controls ovarian cancer cell invasion, downstream of Rab25; Authors - Shengnan Yuan ; KD Validated
human	WB	Nat Commun; Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane; Authors - Tong Chen ; KD Validated
human	IHC	Transl Cancer Res; Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma; Authors - Tianyu Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Shan (Verified Customer) (10-20-2025)

Product came very fast (delivered the next day).

• Applications: Western Blot, Cell culture

FH

Elena (Verified Customer) (05-13-2022)

The antibody detected a single band at the expected molecular weight, under both reducing and non-reducing conditions

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: Ovarian cancer cells