Validation Data Gallery
|Positive WB detected in||HL-60 cells, Apoptosised HeLa cells, human liver tissue, K562, K-562 cells, L02 cells, Jurkat cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human malignant melanoma tissue, human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17817-1-AP targets RAB27A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat, canine, Chicken, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB27A fusion protein Ag12136|
|Full Name||RAB27A, member RAS oncogene family|
|Calculated molecular weight||221 aa, 25 kDa|
|Observed molecular weight||25-27 kDa|
|GenBank accession number||BC107680|
|Gene ID (NCBI)||5873|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.
Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.
Adv Sci (Weinh)
Exosome Release Is Regulated by mTORC1.
J Allergy Clin Immunol
A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.
Cdx2 regulates endo-lysosomal function and epithelial cell polarity.
Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.
Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells.