|Positive WB detected in||HL-60 cells, Jurkat cells, L02 cells, human liver tissue, K562 , K-562 cells, Apoptosised HeLa cells, HeLa cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human stomach tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17817-1-AP targets RAB27A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||canine, Chicken, human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RAB27A fusion protein Ag12136|
|Full Name||RAB27A, member RAS oncogene family|
|Calculated molecular weight||221 aa, 25 kDa|
|Observed molecular weight||25-27 kDa|
|GenBank accession number||BC107680|
|Gene ID (NCBI)||5873|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Rab27a is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27a is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27a is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.
Citrate reduced oxidative damage in stem cells by regulating cellular redox signaling pathways and represent a potential treatment for oxidative stress-induced diseases.
Stem Cells Int
Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats.
Rab27a Contributes to the Processing of Inflammatory Pain in Mice.
J Allergy Clin Immunol
A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.
J Histochem Cytochem
Expression of Secretogranin III in Chicken Endocrine Cells: Its Relevance to the Secretory Granule Properties of Peptide Prohormone Processing and Bioactive Amine Content.