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  • KD/KO Validated

RAB27A Polyclonal antibody

RAB27A Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (4)





Cat no : 17817-1-AP


GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

Tested Applications

Positive WB detected inHL-60 cells, Apoptosised HeLa cells, human liver tissue, K562, K-562 cells, L02 cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman malignant melanoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17817-1-AP targets RAB27A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, rat, Chicken, pig, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB27A fusion protein Ag12136
Full Name RAB27A, member RAS oncogene family
Calculated Molecular Weight 221 aa, 25 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC107680
Gene Symbol RAB27A
Gene ID (NCBI) 5873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.


Product Specific Protocols
WB protocol for RAB27A antibody 17817-1-APDownload protocol
IHC protocol for RAB27A antibody 17817-1-APDownload protocol
IP protocol for RAB27A antibody 17817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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