Tested Applications
| Positive WB detected in | rat brain tissue, HeLa cells, human brain tissue, K-562 cells |
| Positive IP detected in | U-87 MG cells |
| Positive IHC detected in | human colon tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16182-1-AP targets RAB31-specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | RAB31, member RAS oncogene family |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | NM_006868 |
| Gene Symbol | RAB31 |
| Gene ID (NCBI) | 11031 |
| RRID | AB_1851568 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13636 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| IP protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| WB protocol for RAB31-specific antibody 16182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAB31-specific Polyclonal antibody (Cat# 16182-1-AP) has 12 citations published in journals including Cell Research, Journal of Extracellular Vesicles, Journal of Experimental & Clinical Cancer Research, International Journal of Biological Sciences, Life Sciences, Cancer Medicine, Frontiers in Oncology, Frontiers in Cardiovascular Medicine, PLoS One, and FEBS Open Bio. Research fields span extracellular vesicle/exosome biology, cancer (breast, gastric, colorectal, cervical, glioma), cell trafficking, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Baohuoside I chemosensitises breast cancer to paclitaxel by suppressing extracellular vesicle/CXCL1 signal released from apoptotic cells | ||
J Exp Clin Cancer Res Therapeutic synergies that overcome carboplatin resistance in triple-negative breast cancer. | ||
Int J Biol Sci Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.
| ||
Ecotoxicol Environ Saf Lysosome-related exosome secretion mediated by miR-26b / Rab31 pathway was associated with the proliferation and migration of MCF-7 cells treated with BPA | ||
Life Sci Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.
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Reviews
What customers say
The single review (rated 4/5) is from a user who tested this antibody by Western Blot at a 1:500 dilution on K562, KCL22, and THP-1 cell lysates (12 µg protein) with a fluorescent secondary antibody. The user reported that the target "showed up reasonably well," indicating acceptable but not exceptional signal performance under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Edward (Verified Customer) (11-15-2022) | Showed up reasonably well using 12 ug protein lysate with fluorescent secondary
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