RAB31-specific Polyclonal antibody

• 

  WB analysis using 16182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16182-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16182-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16182-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16182-1-AP (RAB31-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16182-1-AP (RAB31-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16182-1-AP (RAB31-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16182-1-AP (RAB31-specific Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

RAB31-specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16182-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

RAB31, RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31

---

Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, K-562 cells
• Positive IHC detected in: human colon tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Product Information

16182-1-AP targets RAB31-specific in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: RAB31, member RAS oncogene family
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: NM_006868
• Gene Symbol: RAB31
• Gene ID (NCBI): 11031
• RRID: AB_1851568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13636
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei ; KD Validated
human	WB	Int J Biol Sci; Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.; Authors - Yujie Huang ; KD Validated
human	WB,IHC,CoIP	Front Oncol; RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.; Authors - Chao-Tao Tang ; KD Validated
human	WB	PLoS One; Human liver cell trafficking mutants: characterization and whole exome sequencing.; Authors - Fei Yuan
mouse,human	WB,IHC,IF	Int J Biochem Cell Biol; Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.; Authors - Chan Qiu ; KD Validated
human	WB,IHC	Life Sci; Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.; Authors - Li Yang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Edward (Verified Customer) (11-15-2022)

Showed up reasonably well using 12 ug protein lysate with fluorescent secondary

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: K562, KCL22 and THP-1