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  • KD/KO Validated

RAB32 Polyclonal antibody

RAB32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10999-1-AP

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32



Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, human colon tissue, HepG2 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10999-1-AP targets RAB32 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB32 fusion protein Ag1450
Full Name RAB32, member RAS oncogene family
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC015061
Gene Symbol RAB32
Gene ID (NCBI) 10981
RRIDAB_2177047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB32 antibody 10999-1-APDownload protocol
IHC protocol for RAB32 antibody 10999-1-APDownload protocol
IF protocol for RAB32 antibody 10999-1-APDownload protocol
IP protocol for RAB32 antibody 10999-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Cell Death Dis

Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission

Authors - Pin Chen
  • KD Validated
mouseWB,IF

mSphere

Burkholderia pseudomallei BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence

Authors - Chenglong Rao
  • KD Validated